After upgrade your Mac to Sierra, it might has some problems after you connected Mac to Arduino (mine one is UNO R3, which works perfect on Capital and older). Something likes this: and you must to restart your machine to it works normal again. So steps to fix are: Remove usb.kext in /System/Library/Extensions/ directory Install a new driver: CH34x_Install_V1.3.pkg Try to plug the Arduino into your USB port again and upload your program!